Fig. 1

Temporal and spatial expression of P4-ATPases and TMEM30A in cochlea. Expression of TMEM30A in the inner hair cells and outer hair cells in the organ of Corti. a–f spiral ganglion neurons (g–i) in Rosenthal’s Canal and stria vascularis (j) in adult mouse cochlea. Enlarged organ of Corti was seen in d–f. Green TMEM30A, Red MyosinVIIa (a–f), Red propidium iodide (PI) (g–j), and blue DAPI. Relative mRNA level of P4-ATPases and Tmem30a at P0 (k), P7 (l), P14 (m), and P30 (n). o The mRNA level of Tmem30a, ATP8A1, ATP8A2, ATP8B1, and ATP11A changed at different postnatal development stages. Error bars indicate the standard error of the mean. OC Organ of Corti, TM tectorial membrane, SV stria vascularis, SL spiral ligament, IHC inner hair cells, OHC outer hair cells, SGN spiral ganglion neurons. In k–o, N = 3