Fig. 4

Disarrangement of kinocilia and stereocilia in TMEM30A knockout HCs. a–f Three rows OHCs and one row IHCs were distinguishable in WT cochlea. The kinocilia indicated by Ac-Tub were on top of the “V-shaped” or “W-shaped” stereocilia (arrows) in OHCs. In the middle turn, the kinocilia were seen in the center of the stereocilia where the small dents were in IHCs. g–l Scattered OHC loss was seen in the apical and middle turn of TMEM30A KO mice. The stereocilia on remaining OHCs lost the characteristic V-shape pattern. Some OHCs were disorientation (arrow head). Kinocilia were hardly recognizable, especially in IHCs. Green shows Ac-Tub, red shows F-actin