Fig. 2
PD-1 knockout and IFN-γ blockade exacerbate inflammation and fibrosis of injured skeletal muscle. WTNS: wild-type injured group with normal saline injection, WTAnti-IFN-γ: wild-type injured group with anti-IFN-γ antibody injection, PNS: PD-1−/− injured group with normal saline injection, PAnti-IFN-γ: PD-1−/− injured group with Anti-IFN-γ antibody injection. A IL-1β mRNA expression at 3 days post injury, B IL-1β mRNA expression at 14 days post injury, C IL-6 mRNA expression at 3 days post injury, D IL-6 mRNA expression at 14 days post injury, E TNF-α mRNA expression at 3 days post injury, F TNF-α mRNA expression at 14 days post injury, G IL-10 mRNA expression at 3 days post injury; H IL-10 mRNA expression at 14 days post injury, I Masson’s trichrome staining of injured TA muscle of mice of both genotypes injected with CTX, along with either anti-IFN-γ antibody or normal saline at 14 days post injury, J Quantification of area of fibrosis as in I, K Col3a1 mRNA expression at 3 days post injury, L Col3a1 mRNA expression at 14 days post injury, M TGF-β mRNA expression at 3 days post injury, N TGF-β mRNA expression at 14 days post injury. *P < 0.05, **P < 0.01, ***P < 0.001, different from NS treatment when comparing the same genotype. #P < 0.05, ##P < 0.01, ###P < 0.001, different from WT mice when comparing the same treatment. Data are shown as mean ± standard deviation (SD), n = 6 (A–H, K–N), n = 3 (J). Green scale bar, 50 μm