Fig. 3
Effects of PD-1 knockout and IFN-γ blockade on macrophage and neutrophil infiltration following injury. WTSham: wild-type uninjured group, PSham: PD-1−/− uninjured group, WTNS: wild-type injured group with normal saline injection, WTAnti-IFN-γ: wild-type injured group with anti-IFN-γ antibody injection, PNS: PD-1−/− injured group with normal saline injection, PAnti-IFN-γ: PD-1−/− injured group with anti-IFN-γ antibody injection. A Mac-2 mRNA expression at 3 days post injury, B F4/80 mRNA expression at 3 days post injury, C CD68 mRNA expression at 3 days post injury, D Mac-2 mRNA expression at 14 days post injury, E F4/80 mRNA expression at 14 days post injury, F CD68 mRNA expression at 14 days post injury, G protein expression levels of Mac-2 and internal control α-tubulin at 3 days post injury, H quantification of Mac-2 expression as in G, I quantification of neutrophils per field as in J, J representative immunofluorescence staining of Ly-6G at 3 days post injury. *P < 0.05, **P < 0.01, ***P < 0.001, different from NS treatment when comparing the same genotype. #P < 0.05, ##P < 0.01, ###P < 0.001, different from WT mice when comparing the same treatment. Data are shown as mean ± standard deviation (SD), n = 6 (A–F), n = 4 (H), n = 3 (I). White scale bar, 50 μm