Fig. 4
IFN-γ blockade after PD-1 knockout increases proinflammatory macrophage activation in injured skeletal muscle. WTNS: wild-type injured group with normal saline injection, WTAnti-IFN-γ: wild-type injured group with Anti-IFN-γ antibody injection, PNS: PD-1−/− injured group with normal saline injection, PAnti-IFN-γ: PD-1−/− injured group with Anti-IFN-γ antibody injection. A Representative double immunofluorescence staining of iNOS and Mac-2 at 3 days post injury, B quantification of pro-inflammatory macrophages per field as in A, C iNOS mRNA expression at 3 days post injury, D iNOS mRNA expression at 14 days post injury, E CD86 mRNA expression at 3 days post injury, F CD86 mRNA expression at 14 days post injury. *P < 0.05, ***P < 0.001, different from NS treatment when comparing the same genotype. #P < 0.05, ###P < 0.001, different from WT mice when comparing the same treatment. Data are shown as mean ± standard deviation (SD), n = 3 (B), n = 6 (C–F). White scale bar, 50 μm. → Proinflammatory macrophage