Fig. 5
IFN-γ blockade after PD-1 knockout reduces anti-inflammatory macrophage activation in injured skeletal muscle. WTNS: wild-type injured group with normal saline injection, WTAnti-IFN-γ: wild-type injured group with Anti-IFN-γ antibody injection, PNS: PD-1−/− injured group with normal saline injection, PAnti-IFN-γ: PD-1−/− injured group with Anti-IFN-γ antibody injection. A Representative double immunofluorescence staining of Arg1 and Mac-2 at 3 days post injury, B representative double immunofluorescence staining of Arg1 and Mac-2 at 14 days post injury, C quantification of anti-inflammatory macrophages per field as in A, D quantification of anti-inflammatory macrophages per field as in B, E Arg1 mRNA expression at 3 days post injury, F Arg1 mRNA expression at 14 days post injury, G CD206 mRNA expression at 3 days post injury, H CD206 mRNA expression at 14 days post injury. *P < 0.05, **P < 0.01, ***P < 0.001, different from NS treatment when comparing the same genotype. #P < 0.05, ##P < 0.01, ###P < 0.001, different from WT mice when comparing the same treatment. Data are shown as mean ± standard deviation (SD), n = 3 (C, D), n = 6 (E–H). White scale bar, 50 μm. → Anti-inflammatory macrophage