Fig. 3

LAMP3 is significantly correlated with RPL21 and promotes the migration and invasion of CRC. A Heatmap of the top 20 differentially expressed genes in the transcriptome sequencing of RPL21 stable overexpression and knockdown cells with their control cells. B LAMP3 protein expression in RPL21 stable overexpression and knockdown cells detected by western blotting. C The representative images of RPL21 and LAMP3 expression in 142 paraffin-embedded human CRC tissues (tumor 1, tumor 2, and tumor 3) detected by IHC staining (scale, 100 μm). D The representative image of LAMP3 expression in CRC tumor buds was detected by IHC staining (scale, 100 μm). The arrowheads indicate the tumor buds. The correlation between the scores of LAMP3 expression in tumor buds and the grades of tumor budding was analyzed. n = 142, ***p < 0.001, Kendall’s Tau test. E The correlation between the scores of RPL21 and LAMP3 expression in tumor buds was analyzed. n = 142, ***p < 0.001, Kendall’s Tau test. F The migration ability of the indicated CRC cells was detected by the transwell migration assay (scale, 100 μm). Mean ± SD, n = 5, ***p < 0.001, Student’s t-test. G The invasion ability of indicated CRC cells was detected by Matrigel-coated Boyden chamber invasion assay (scale, 100 μm). Mean ± SD, n = 5, **p < 0.01, Student’s t-test