Fig. 6

RPL21–LAMP3 regulates FA formation and promotes cell spreading. A KEGG enrichment analysis of the RPL21 expression in the CRC database GSE39582. B The adhesion ability of the indicated cells was detected by the FN adhesion assay (scale, 100 μm). Mean ± SD, n = 5, ***p < 0.001, **p < 0.01, *p < 0.05, Student’s t-test. C The morphology of FA (paxillin, green) of the indicated cells was observed by IF staining (scale, 20 μm). D FAK, pTyr397-FAK, paxillin, pTry118-paxillin, ERK1/2, pThr202/Tyr204-ERK1/2, RPL21, and LAMP3 protein expression in the indicated CRC cells was detected by western blotting