Fig. 2

KIAA1429 promoted cell proliferation, inhibited apoptosis, and facilitated cell cycle progression in DLBCL. A Dendrogram showing hierarchical clustering of genes in distinct modules. B, C GO and KEGG analyses of genes correlated with KIAA1429 in DLBCL according to the GEO database (GSE117556). D, E RT-qPCR and western blotting analyses verified the knockdown effect of KIAA1429 at the mRNA and protein levels, respectively. At least three independent experiments were conducted to obtain the data presented as mean ± SD. **p < 0.01; ***p < 0.001; ****p < 0.0001. F Knockdown of KIAA1429 inhibited DLBCL cell proliferation as determined by CCK-8 assay. Triplicate data are presented as mean ± SD. *p < 0.05; ***p < 0.001; ****p < 0.0001. G Cell apoptosis was suppressed by knockdown of KIAA1429. Left: representative results; Right: statistical data of three independent experiments are shown as mean ± SD. **p < 0.01. H Induction of cell cycle arrest in G2/M phase upon KIAA1429 knockdown detected by flow cytometry. Left: representative results; Right: as a result of three independent experiments, mean ± SD are shown. ***p < 0.001; ****p < 0.0001. I, J Endogenous KIAA1429 overexpression by the CRISPR/dCas9-VP64 gene activation system was demonstrated at both the mRNA and protein levels. (NTC represents nontarget control; KIAA1429 OE represents overexpression of KIAA1429). Mean ± SD is shown for three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001. K The cell proliferation rate was enhanced by overexpression of KIAA1429 as detected by CCK8 assay. An average of three independent experiments is represented as mean ± SD. **p < 0.01; ****p < 0.0001. L, M Overexpression of KIAA1429 reduced cell apoptosis and modulated the expression of apoptosis-related proteins. Top: representative results; bottom: data from three independent experiments are presented as mean ± SD. **p < 0.01. N, O Cell cycle distribution and the regulation of cell cycle-related proteins upon KIAA1429 overexpression are displayed. Top: representative results; bottom: data are represented as mean ± SD. *p < 0.05; **p < 0.01