Fig. 3

Deletion of KIAA1429 in DLBCL cells exerted antitumor effects both in vitro and in vivo. A, B Stable knockout of KIAA1429 in DLBCL cells by CRISPR/Cas9 was confirmed by RT-qPCR and western blotting. (WT represents wild-type cells; KIAA1429 KO represents CRISPR/CAS9 knockout cells). Three independent experiments were performed to obtain the data presented as mean ± SD. **p < 0.01; ****p < 0.0001. C Knockout of KIAA1429 dramatically suppressed the proliferation of DLBCL cells as determined by CCK8 assay. Three independent experiments are presented as mean ± SD. ***p < 0.001; ****p < 0.0001. D, E KIAA1429 deletion facilitated apoptosis and affected the expression of apoptosis-related proteins. Top: representative results; bottom: data from three independent experiments are represented as mean ± SD. **p < 0.01; ***p < 0.001. F, G The cell cycle was arrested in G2/M phase followed by alterations in cell cycle-associated proteins upon KIAA1429 knockout. Top: representative results; bottom: statistical results from three independent experiments are represented as mean ± SD. *p < 0.05; **p < 0.01. H, I SCID beige mice were subcutaneously injected with OCI-LY1 cells with or without KIAA1429 deletion (n = 6), and tumor sizes and volumes were decreased in KIAA1429 knockout cells. **p < 0.01. J Histological analysis of excised tumors by hematoxylin/eosin (HE) staining and Ki67. Bar, 50 μm