Fig. 6

CHST11 functioned as a tumor suppressor in DLBCL. A Knockdown of CHST11 promoted cell proliferation in DLBCL. Mean ± SD is presented for three independent experiments. **p < 0.01; ***p < 0.001; ****p < 0.0001. B The apoptosis rate was reduced in CHST11 knockdown cells compared to control cells. Left: representative results; right: statistical data are presented as mean ± SD based on three independent experiments. ****p < 0.0001. C Cell cycle distribution of control and CHST11-deficient DLBCL cells was determined by flow cytometry. Left: representative results; right: data are expressed as mean ± SD from three independent experiments. *p < 0.05. D, E OCI-LY1 cells with or without CHST11 depletion were injected subcutaneously into SCID beige mice (n = 7), and CHST11 knockdown cells presented increased tumor size and tumor volume. F Histological analysis of resected tumors was performed by hematoxylin/eosin (HE) staining and Ki67 staining. Bar, 50 μm. G Cell proliferation was assessed in DLBCL cells with or without KIAA1429 knockdown or combined KIAA1429 knockdown and CHST11 knockdown. Data from three independent experiments are presented as mean ± SD. **p < 0.01; ***p < 0.001; ****p < 0.0001. H, I Examination of apoptosis and the expression of apoptosis-related proteins in DLBCL cells with or without KIAA1429 knockdown or in combination with KIAA1429 knockdown and CHST11 knockdown. Left: representative results; Right: data from three independent experiments are represented as mean ± SD. ***p < 0.001; ****p < 0.0001. J, K Flow cytometry was used to detect the distribution of cell cycle in DLBCL cells with or without KIAA1429 knockdown or combined KIAA1429 knockdown and CHST11 knockdown. Left: representative results; Right: data are shown as mean ± SD from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001