Fig. 7

KIAA1429 inactivated Hippo–YAP signaling by interacting with CHST11. A GO enrichment analysis showed high enrichment of the Hippo signaling pathway based on RNA-seq. B A positive correlation between CHST11 and MOB1B was found in Pearson correlation analysis. C The binding between CHST11 and MOB1B proteins was confirmed by co-IP in OCI-LY1 and U2932 cells. D Colocalization of CHST11 and MOB1B protein in OCI-LY1 and U2932 cells was exhibited in confocal immunofluorescent images. Bar, 20 μm. E The protein level of MOB1B was detected in DLBCL cells with or without KIAA1429 knockdown or with combined KIAA1429 knockdown and CHST11 knockdown. F Expression of core proteins in the Hippo pathway was examined in DLBCL cells with or without KIAA1429 knockdown or with combined KIAA1429 knockdown and CHST11 knockdown. G Knockdown of CHST11 suppressed MOB1B protein expression and LATS1 phosphorylation. H YAP expression was detected by western blotting after isolation and extraction of cytoplasmic and nuclear proteins. I Endogenous expression and subcellular localization of YAP were assessed by immunofluorescence staining. Bar = 20 μm. Data were obtained from three independent experiments. J Western blotting showed that knockdown of CHST11 rescued the KIAA1429 knockdown-induced decrease in YAP and TEAD4 expression and increase in YAP phosphorylation