Fig. 1
From: Optogenetic control of YAP can enhance the rate of wound healing
Characterisation of optoYAP in H9c2 cells. A Representative images of H9c2 cells transfected with optoYAP kept in the dark (t = 0 min), then subjected to pulsatile light activation for 20 min (t = 20 min), followed by recovery in the dark for a further 20 min (t = 40 min). Scale bar, 20 µm. B Quantification of activation (τa) and recovery (τr) time constant of optoYAP. Red line represents average nuclear/cytoplasmic ratio. Vertical dashed line represents time when 488 nm stimulation ceased. Numbers in brackets represent the 95% confidence interval of τ. n = 12 cells. (C) RT-qPCR of YAP downstream genes. Cells were transfected with optoYAP, and transcript levels were assessed after 48 h of activation. Expression levels of CTGF, CYR61, and TGF-β were normalised to the housekeeping gene EIF1B and described as relative fold change. Horizontal bars represent mean and 95% confidence interval from 6 biological replicates from two independent experiments. NS not significant, *P < 0.05, **P < 10–2, ***P < 10–3