Fig. 4
From: Optogenetic control of YAP can enhance the rate of wound healing
Point mutations of optoYAP at ERK phosphorylation serine residues. A Three mutants containing phosphomimetic of ERK target residues at S251, S333 or both S251 and S333. Representative images of HEK293T cells transfected with different mutant optoYAP before and after exposure to pulsatile light activation. Scale bars, 10 µm. B Quantification of cells from (A), measuring nuclear to cytoplasmic ratio of optoYAP before and after light activation for each mutant. Box plots represent median and 25th to 75th percentiles. Whiskers show minimum and maximum points. NS: not significant, **P < 10–2. C Fraction increase of N/C ratio after light activation. Bar represents average and error bars represent s.d. D Relative N/C ratio over time during 488 nm activation amongst optoYAP mutants. E Absolute intensity values of mCherry signal in nuclei over time. Solid lines represent mean, n = 10 cells taken from three independent experiments