Fig. 5
From: Optogenetic control of YAP can enhance the rate of wound healing
optoYAP variants under light activation. Representative images of mCherry signal in HEK293T cells containing A wildtype (WT) optoYAP, B optoYAP with a point mutation at S127A, or C truncated optoYAP without the C-terminal PDZ-binding motif (optoYAP ΔC). The ‘Pre activation’ panels show cells before exposure to 488 nm light, and after 20 min of pulsatile activation with 488 nm light, the same cells were imaged as ‘Post activation’. Cells were left in the dark for a 20 min and imaged again, under the ‘Recovery’ panel. D Quantification of nuclear to cytoplasmic ratio of optoYAP in (A–C). Box plots represent median and 25th to 75th percentiles. Whiskers show minimum and maximum points. Scale bars, 10 µm. n = 22 cells for (A), n = 10 cells for (B, C), taken from three independent experiments. *P < 0.05, ***P < 10–3. E, F Quantification of activation (τa) and recovery (τr) time constant of E S127A mutant and F ΔC mutant during light activation and recovery. Red line represents average and vertical dashed line represents time when 488 nm stimulation ceased. Numbers in brackets represent the 95% confidence interval of τ. n = 8 cells