Table 1 List of primers for plasmid cloning
From: Optogenetic control of YAP can enhance the rate of wound healing
Primer name | Sequence | Cloning | Restriction enzyme |
|---|---|---|---|
PT25 hYAP1-1delta fwd | ATTTATGGTACC ATGGATCCCGGGCAG | Forward primer for S127A and ΔC mutants | KpnI |
PT26 hYAP1-1delta S127A rev | CGCGCCGTTTAAACC TATAACCATGTAAG | Reverse primer for S127A | PmeI |
PT27 hYAP1-1delta DeltaC rev | CGCGCCGTTTAAACC TAGCTTTCTTTATCTAGC | Reverse primer for ΔC | PmeI |
795-2194-YAP-S251D-F1 | GATCCACAGGGAGG CGTCATGGGTG | Forward primer for SDM of S251D mutant | – |
796-2194-YAP-S251D-R1 | CTGGGGAGCCAGGG GTGGTGGCTGT | Reverse primer for SDM of S251D mutant | – |
797-2195-YAP-S333D-F1 | GATCCCGGGATGTC TCAGGAATTGAG | Forward primer for SDM of S333D mutant | – |
798-2195-YAP-S333D-R1 | CGACACTGGATTTT GAGTCCCACCATC | Reverse primer for SDM of S333D mutant | – |
799-2196-YAP-2mut-F1 | CAGCCACCACCCCTGGC TCCCCAGGATCCACAGG GAGGCGTCATGGGTG | Forward primer for SDM of S251D and S333D mutant | – |
800-2196-YAP-2mut-R1 | CTCAATTCCTGAGACATC CCGGGATCCGACACTGG ATTTTGAGTCCCACCATC | Reverse primer for SDM of S251D and S333D mutant | – |