Fig. 1
The expression and characteristics of circRNF10 in BC. A Volcano plot showing the differentially expressed circRNAs in BC in GSE101123 dataset. The cutoff was set at |log2(fold change)| > 1 and P < 0.05. B Box plot showing the expression of circRNF10 in mammary gland and BC according to GSE101123 dataset. C RT-qPCR analysis of circRNF10 expression in BC cells compared with MCF-10A cells. D RT-qPCR analysis of circRNF10 expression in BC tissues presented as the ratio of tumor/adjacent (N = 108). E RT-qPCR analysis of circRNF10 expression in BC tissues and adjacent tissues (N = 108). F Kaplan-Meier (K–M) plot presenting PFS of high and low circRNF10 expression group. G The formation of circRNF10 confirmed by Sanger sequencing. H RT-PCR analysis of circRNF10 and linear RNF10 with divergent and convergent primers in MDA-MB-231 and MCF-7 cells. I The expression of circRNF10 after actinomycin D treatment in MDA-MB-231 and MCF-7 cells. J The expression of circRNF10 after RNase R treatment in MDA-MB-231 and MCF-7 cells. K Subcellular fractionation analysis of circRNF10, with U6 and GAPDH as enteral control of the nucleus and cytoplasm. L FISH analysis of circRNF10 localization in MDA-MB-231 and MCF-7 cells. Red: circRNF10, blue: DAPI. Error bars represent the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001