Fig. 2

CircRNF10 was a tumor suppressor in BC cells. A RT-qPCR analysis of si-circRNF10 efficiency in BC cells. B RT-qPCR analysis of lentivirus (LV)-circRNF10 efficiency in BC cells. C Colony formation assay performed in si-NC and si-circRNF10 groups to evaluate the effect of inhibiting circRNF10 in BC cells (left) and the statistics of colony numbers (right). D Colony formation assay performed in LV-vector and LV-circRNF10 groups to evaluate the effect of overexpressing circRNF10 in BC cells (left) and the statistics of colony numbers (right). E MTT assay showing the proliferation of BC cells in si-NC and si-circRNF10 groups. F MTT assay showing the proliferation of BC cells in LV-vector and LV-circRNF10 groups. G Wound-healing assay comparing the migration of MDA-MB-231 cells in si-circRNF10 group with control (left) and the statistics of wound closure percentage (right). H Wound-healing assay comparing the migration of MDA-MB-231 cells in LV-circRNF10 group with control (left) and the statistics of wound closure percentage (right). I Transwell assay showing the migration of MDA-MB-231 cells transfected with si-NC or si-circRNF10 (left) and the number of migrated cells (right). J Transwell assay showing the migration of MDA-MB-231 cells expressing LV-vector or LV-circRNF10 (left) and the number of migrated cells (right). Error bars represent the means± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001