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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: CircRNF10-DHX15 interaction suppressed breast cancer progression by antagonizing DHX15-NF-κB p65 positive feedback loop

Fig. 5

CircRNF10 inhibited BC progression by antagonizing DHX15-p65 interaction. A Co-IP assay detecting the interaction between endogenous DHX15 and p65 in BC cells. B Western blotting analysis presenting the influence of LV-DHX15 on the p-p65 (S536) levels in total protein and the cytoplasmic and nuclear p65 levels, when p65 was suppressed in BC cells. C Western blotting analysis presenting the influence of si-DHX15 on the p-p65 (S536) levels in total protein, and the cytoplasmic and nuclear p65 levels when BC cells were treated with LPS (250 ng/ml). D Co-IP assay presenting the change of DHX15-p65 interaction after inhibiting circRNF10 in BC cells. E MTT assay showing the influence of circRNF10 overexpression on the proliferation of DHX15 overexpressed MDA-MB-231 cells. F MTT assay showing the influence of circRNF10 overexpression on the proliferation of DHX15 overexpressed MCF-7 cells. G Colony formation assay showing the effect of circRNF10 overexpression on the proliferation of DHX15 overexpressed BC cells. H The number of colonies. Error bars represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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