Fig. 6

DHX15-p65 positive feedback loop in BC was impaired by circRNF10. A Correlation between DHX15 and RELA expression in BC according to the GEPIA2 database. B RT-qPCR analysis showing the efficiency of si-RELA in BC cells. C RT-qPCR analysis showing the efficiency of OE-RELA in BC cells. D RT-qPCR analysis showing the expression of DHX15 after suppressing RELA. E RT-qPCR analysis showing the expression of DHX15 after overexpressing RELA. F Western blotting assay presenting the levels of DHX15 and p65 in si-NC and si-RELA groups of BC cells. G Western blotting assay presenting the levels of DHX15 and p65 in vector and OE-RELA groups of BC cells. H Schematic illustration showing the predicting of p65 binding sites on DHX15 promoter region. I Schematic illustration showing the reporter plasmids cloning DHX15 promoter region with different length (left) and dual-luciferase assay detecting the binding of p65 and DHX15 promoter region (right). J ChIP assay showing that p65 directly binds to the promoter region of DHX15 by agarose gel electrophoresis (left) and qPCR (right). K EMSA suggesting that the complexity of p65 and DHX15 promoter existed. L Western blotting assay showing the expression of DHX15 after inhibiting circRNF10 in BC cells. M Western blotting assay showing the expression of DHX15 after overexpressing circRNF10 in BC cells. Error bars represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001