Comparison between 5 extractions methods in either plasma or serum to determine the optimal extraction and matrix combination for human metabolomics

Table 1 Repeatability of extraction methods in plasma and serum assessed by global metabolomics analysis

		Mean RSD % (HILIC +)	Mean RSD % (HILIC −)	Mean RSD % (C18 +)	Mean RSD % (C18 −)	Global Mean RSD %
PLASMA	MeOH	30	24	27	19	25
	MeOH:ACN	21	32	20	24	24
	ACN	26	37	22	68	38
	Hybrid-SPE/ACN	44	46	30	35	39
	Hybrid-SPE/MeOH	51	46	43	38	45
SERUM	MeOH	30	43	27	19	30
	MeOH:ACN	36	41	24	39	35
	ACN	36	80	43	74	58
	Hybrid-SPE/ACN	45	38	26	56	41
	Hybrid-SPE/MeOH	47	100	47	50	61

The table presents the average RSD (%) computed for all potential metabolite features identified in plasma and serum through the use of five extraction techniques. The RSD for each potential metabolite feature was determined by examining the raw signal intensities from extraction replicates (n = 3). The number of features with RSD ≤ 30% (between replicates) represents high quality features that could be applied for identifying biomarkers and conducting pathway analysis in global metabolomics studies. MeOH methanol, MeOH:ACN methanol combined with acetonitrile, ACN acetonitrile, SPE solid preparation extraction, RSD relative standard deviation

ISSN: 1689-1392