Fig. 2

Mechanical stretching altered oxidative pattern in PDLSCs. A The relative RNA expression of LDH and COX4 in PDLSCs after stretching for 0, 6, 12, and 24 h. B The protein level of LDH and COX4 in PDLSCs after stretching for 0, 6, 12, and 24 h. C The expression of LDH and COX4 in PDLSCs stretched for 0 h and 24 h visualized by immunofluorescence staining. Scale bar = 50 μm. D The level of lactic acid produced by PDLSCs after stretching for 0, 6, 12, and 24 h. E, F The level of ROS in PDLSCs after 0, 6, 12, and 24 h of stretching. Scale bar = 100 μm. **P < 0.01, *P < 0.05, ***P < 0.001, ns no significance