Fig. 5

Mechanical force-led alteration of oxidation pattern in PDLSCs was induced by mitophagy. A The protein level of MFF, p-MFF, PINK1, p-PINK1, PARKIN, and p-PARKIN in PDLSCs after stretching for 0, 6, 12, and 24 h. B The morphology of mitochondria in CsA pretreated PDLSCs after 0 h and 24 h of stretching visualized by TEM. Red arrow: mitochondria. Scale bar = 1 μm. C The MMP in CsA pretreated PDLSCs after stretching for 0 h and 24 h visualized by TMRM staining. Scale bar = 100 μm. D The relative RNA expression of LDHA and COX4 in CsA pretreated PDLSCs after being stretched for 0 h and 24 h. E The protein level of LDH and COX4 in CsA pretreated PDLSCs after being stretched for 0 h and 24 h. F The level of lactic acid in CsA pretreated PDLSCs after stretching for 0 h and 24 h. G The level of ROS in CsA pretreated PDLSCs after stretching for 0 h and 24 h. Scale bar = 100 μm. *P < 0.05. ns no significance