Table 1 The primers and antibodies used in this study
From: Mechanical force induces mitophagy-mediated anaerobic oxidation in periodontal ligament stem cells
Primers | |
 LDHA | Forward: TTGTTGGGGTTGGTGCTGTTG |
Reverse: AAGAGCAAGTTCATCTGCCAAG | |
 COX4 | Forward: CGATCACCTTGACGGACGAG |
Reverse: TCTTCTCATAGTCCCAGCGC | |
Antibodies for western blot | |
 LDH | Abcam, anti-lactate dehydrogenase antibody, ab52488 |
 COX4 | CST, COX IV (3E11) rabbit mAb, #4850 |
 DRP1 | Proteintech, DRP1 polyclonal antibody, 12957-1-AP |
 MFN2 | Proteintech, MFN2 polyclonal antibody, 12186-1-AP |
 MFF | Abcam, anti-MFF antibody, ab129075 |
 PINK1 | Proteintech, PINK1 polyclonal antibody, 23274-1-AP |
 PARKIN | Proteintech, PARK2/Parkin monoclonal antibody, 66674-1-Ig |
 GAPDH | Proteintech, GAPDH polyclonal antibody, 10494-1-AP |
 β-actin | Proteintech, beta actin polyclonal antibody, 20536-1-AP |
Antibodies for immunofluorescence staining | |
 LDH | Abcam, anti-lactate dehydrogenase antibody, ab52488 |
 COX4 | CST, COX IV (3E11) rabbit mAb, #4850 |
ldh | Abcam, anti-lactate dehydrogenase antibody, ab52488 |
 Cox4 | CST, COX IV (3E11) rabbit mAb, #4850 |
 DRP1 | Proteintech, DRP1 (C-terminal) polyclonal antibody, 12957-1-AP |
 VDAC1 | Proteintech, VDAC1/Porin monoclonal antibody, 66345-1-Ig |
 Drp1 | Proteintech, DRP1 (C-terminal) polyclonal antibody, 12957-1-AP |
 Vdac1 | Proteintech, VDAC1/Porin monoclonal antibody, 66345-1-Ig |
Antibodies for immunohistochemical staining | |
 ldh | Abcam, anti-lactate dehydrogenase antibody, ab52488 |
 cox4 | CST, COX IV (3E11) rabbit mAb, #4850 |