Fig. 2

Editing specificity comparison of the CRISPR-Cas12a systems with different promoters. a Schematic of the editing specificity analysis by Tag-seq among the CRISPR-Cas12a systems with different promoters. b Tag-seq-based comparative analysis of CRISPR-Cas12a systems with different promoters (also see Additional file 2: Fig. S2). For visualization, the crRNA sequences were shown at the top, and the on-target and off-target sites were shown without or with mismatches to the crRNA sequence by color highlighting. Sequencing read counts were shown to the right of each site. c Normalization of on-target activity of the various CRISPR-Cas12a systems to the CRISPR-Cas12a driven by the CAG promoter, value = (other systems on-target reads)/(CRISPR-Cas12a with CAG promoter). d Total number of off-target sites detected with the twenty-five crRNAs. e Specificity Index assessment (value was calculated by the ratio of total on-target reads to the on-target reads plus the off-target reads within the 25 sites)