Fig. 3

Multiplex-editing specificity comparison of the CRISPR-Cas12a systems with different promoters. a Schematic of the multiplex editing. b Tag-seq-based comparative analysis of CRISPR-Cas12a systems with different promoters in multiplex editing. c Normalization of on-target activity of the various CRISPR-Cas12a systems to the CRISPR-Cas12a driven by the CAG promoter in multiplex editing, value = (other system on-target reads)/(CRISPR-Cas12a with CAG promoter). d Total number of off-target sites detected with the six crRNAs in multiplex editing. e Specificity Index assessment (value was calculated by the ratio of total on-target reads to the on-target reads plus the off-target reads within the six sites)