Fig. 5
From: Profiling the impact of the promoters on CRISPR-Cas12a system in human cells
Base editing comparison of the CRISPR-Cas12a systems with different promoters. a Schematic of the base editing systems based on CRISPR-Cas12a with different promoters. rAPOBEC1, rat APOBEC1. b Sanger sequencing analysis of the base editing ability at FANCF locus. c Deep-seq revealed the cytosine to thymine (C-to-T) editing at FANCF site. Mean values are presented with SEM, n = 3 independent experiments. d Analysis of the editing purity at site 3. The fraction was plotted by calculating each nucleotide read within the total reads at this site. Mean values are presented with SEM, n = 3 independent experiments. NC, cells transfected with a non-targeted crRNA