From: Profiling the impact of the promoters on CRISPR-Cas12a system in human cells
Contents | Promoter | |||
---|---|---|---|---|
CAG | EF1a Core | CMV | PGK | |
Size | ~1700 bp | ~210 bp | ~500 bp | ~500 bp |
GC content | ~66 % | ~59 % | ~49 % | ~65 % |
Activitya | 100% | 40–60% | 70–90% | 40–60% |
Specificityb | ~75% | ~84% | ~78% | ~82% |
Off-target No. (in 25 crRNAs ) | 19 | 14 | 18 | 14 |
Application scenarios | Need robust activity without size limitation | Require a compact size design | An alternative for CAG | Require a lower level of Cas-protein |