Fig. 2

Cd36-KO mice exhibit similar T cell phenotypes and in vitro expansion compared with WT mice. A Representative flow cytometry of the lymphocytes population from mouse spleen cells stained with CD3, CD4, CD8, and CD25 flow antibody to evaluate the different T cell population in WT mice and Cd36-KO mice. Quantification results of the positive cell population percentages were represented by the bar graph, in which each bar represents the mean with standard error of population percentage for Cd36-KO and WT mice (n = 6 mice per group). B A continuous 10-day proliferation assay was performed on healthy mouse primary spleen cells (n = 3 biological replicates). The cell numbers among WT and Cd36-KO groups were normalized to the first day and then were compared. Unpaired t-test was used to analyze the fold change difference. (ns, not significant). C The flow cytometry analysis demonstrated that CellTrace CFSE fluorescent stains from permanently labeled parent cells were diluted through subsequent cell divisions, and reduced fluorescent intensities were similar in daughter cells between WT and Cd36-KO mice spleen cells (n = 3 biological replicates). The bar graph showed similar T cell expansion in splenocytes between WT and Cd36-KO mice by comparing the mean of fluorescence intensity with standard error on day 1, 3, 5, 7, and 9. Unpaired t-test was used to analyze the percentage difference (ns, not significant)