Fig. 3

Loss of Cd36 is associated with reduced hematopoietic stem and progenitor cells colony formation. A Representative flow cytometry result showing the stem and progenitors as a percentage of ckit and Il7rα fresh and enriched BM cells collected from both WT mice and CD36-KO mice (n = 4 female mice per group); early form of murine hematopoietic stem cell (KLS), common lymphoid progenitor (CLP), common myeloid progenitor (CMP), megakaryocyte-erythroid progenitor (MEP), and granulocyte–macrophage progenitor (GMP). Quantification results of the positive cell population percentages were represented by the bar graph, in which each bar represents the mean with standard error of population percentage for Cd36-KO and WT mice (n = 4 mice per group). The differences between groups were analyzed using unpaired t-test (ns, not significant). B A continuous 10-day proliferation assay was performed on BM cells (n = 3 biological replicates). The cell numbers among WT and Cd36-KO groups were normalized to the first day and then were compared. Unpaired t-test was used to analyze the fold change difference. (ns, not significant). C Flow cytometry analysis demonstrated that CellTrace CFSE fluorescent stains from permanently labeled parent cells were diluted through subsequent cell divisions, and the reduced fluorescent intensities were similar in daughter cells between WT and Cd36-KO mice BM cells (n = 3 biological replicates). The bar graph showed similar HSPCs expansion in BM between WT mice and Cd36-KO mice by comparing the mean of fluorescence intensity with standard error on day 1, 3, 5, 7, and 9. Unpaired t-test was used to analyze the percentage difference. (ns, not significant). D The colony forming units (CFUs) were manually counted for each replicate in both Cd36-KO and WT groups, and then normalized relative to the mean counts of WT group. The colored bar graph represents mean with standard error of CFU count for WT and Cd36-KO mice group. Each colored dot represents the count of each single replicate (n = 8 biological replicates). The difference between WT and Cd36-KO group was analyzed by Mann–Whitney test (***, P < 0.001)