Fig. 3
From: Galectin-1 promotes angiogenesis and chondrogenesis during antler regeneration
Effects of deer GAL-1 on angiogenesis and chondrogenesis. A, B The proliferation of HUVECs exposed to APCs-cultured medium was measured by Cell CCK8 (A; 24, 48, 72, and 96 h) and Edu assay (B; 48 h), respectively. C, D The proliferation of HUVECs exposed to exogenous deer GAL-1 (20 ng/ml) was measured by CCK8 (C; 24, 48, 72, and 96 h) and Edu assay (D; 48 h), respectively, as compared with control wells without deer GAL-1. E, F Tube formation assay of HUVECs exposed to APCs-cultured medium (E) and deer GAL-1 recombinant protein (F), respectively. G, H Migration assay of HUVECs exposed to APCs-cultured medium (G) and deer GAL-1 recombinant protein (H), respectively. I Effects of GAL-1 gene knocked out on chondrogenic differentiation of APCs. Chondrogenic differentiation of APCs grown as micro-mass cultures. Chondrogenic pellets were cut into 5 μm sections for Alcian blue staining and immunohistochemical staining for type II collagen and GAL-1. The Alcian Blue and Collagen type II protein staining depth of the resultant nodules were decreased in the APCGAL-1−/− compared with the control. The results showed that APCGAL-1−/− did not form cartilaginous nodules compared to the control group. Data are presented as the mean ± SD, t-test or two-way ANOVA test (CCK8 assay), *P < 0.05, **P < 0.01, ***P < 0.001