Fig. 4

A general view of gene expression patterns and differentially expressed genes (DEGs) analysis on APC^WT, APC^Vector, and APC^GAL-1−/−. A The PCA analysis was performed on the transcript signatures of APC^WT, APC^Vector, and APC^GAL-1−/−. B–D The volcano plots showed the expression levels of DEGs between APC^WT and APC^Vector (B), between APC^GAL-1−/− and APC^WT (C), and between APC^GAL-1−/− and APC^Vector (D), respectively. Y-axis denotes the -log10 value of the adjusted P-value (padj) of DEGs, padj < 0.01. The X-axis represents the log2foldchange values of the DEGs. The values > 1 are marked by pink, representing the up-regulated genes of APC^GAL-1−/−, and the values < − 1 are marked by blue, representing the down-regulated genes of APC^GAL-1−/−.The values > − 1 and < 1 are marked by grey color. E Venn analysis was performed to select the co-expressed DEGs between the APC^GAL-1−/− vs. APC^WT and APC^GAL-1−/− vs. APC^Vector. DEGs differentially expressed genes. F, G Verification of the transcriptome results on the selected DEGs by qRT-PCR.