Fig. 1

Empagliflozin pretreatment protected IRI mice from kidney injury. Empagliflozin was injected through the caudal vein in advance in empagliflozin-treated mice. a The renal pathological changes were presented by HE staining. Then, renal cell injury was quantified as injury scores. b TUNEL fluorescence assay was used to detect apoptosis and the strength of fluorescent signals presented apoptosis intensity. DAPI was used to label the nuclei. The colocalization of TUNEL and nuclei showed their positional relationships. c We detected the expression of caspase3, Bax, OPA1, and Mfn1 via western blot and used β-actin as the internal reference. The experiments were repeated independently at least three times, and the data were presented as the means ± SEM. Statistical significance with respect to the control was marked with *P < 0.05, **P < 0.01, or ***P < 0.001