Fig. 3

Empagliflozin effectively inhibited the shortening of mitochondria and promoted the expression of OPA1 in HK-2 cells under H/R. a Mito-Tracker Red CMXRos was used to mark mitochondria with bioactivity specifically. DAPI was used to label the nuclei. The colocalization of mitochondria and nuclei showed their positional relationships. b TOMM20 pAb was used to detect levels of TOMM20 protein. DAPI was used to label the nuclei. The colocalization of mitochondria and nuclei showed their positional relationships. c We detected the expression of OPA1 via immunofluorescence. d The expression of Mfn1, OPA1, and Mff was then detected via western blot, using β-actin as the internal reference. The results were subsequently quantified. The experiments were repeated independently at least three times, and the data were presented as the means ± SEM. Statistical significance with respect to control was marked with *P < 0.05, **P < 0.01 or ***P < 0.001