Fig. 4

Empagliflozin positively protected mitochondria in an OPA1-knockdown, IRI-like condition in HK-2 cells. a Mito-Tracker Red CMXRos was specifically used to mark mitochondria with bioactivity. DAPI was used to label the nuclei. The colocalization of mitochondria and nuclei showed their positional relationships. Mitochondrial morphology was then quantified. b The mitochondrial length was detected via immunofluorescence. TOMM20 pAb was used to detect levels of TOMM20 protein. DAPI was used to label the nuclei. The colocalization of mitochondria and nuclei showed their positional relationships. The experiments were repeated independently at least three times, and the data were presented as the means ± SEM. Statistical significance with respect to controls was marked with *P < 0.05, **P < 0.01, or ***P < 0.001