Fig. 2

Notch signaling induced the glycolytic switch as the dominant energy supply mode during the mid–late embryonic stages. A Developmental changes of Notch signaling in the liver of geese embryos from E22, E25, and E28 (n = 6). B Correlation between hepatic glycolysis and Notch signaling. C Schematic depiction of Notch signaling regulating glycolysis in vivo and in vitro. D Effect of in ovo injection with 20 mg/kg 2-DG or 10 nM DAPT on lactate and ATP content in the liver (n = 6). E, F Effect of in ovo injection with 20 mg/kg 2-DG or 10 nM DAPT on mRNA expression of Hey1 (E), HK2, PFK, PKM, and LDHA (F) in the liver (n = 6). G–I Protein abundances of Hey1 and NICD (G, H) as well as HK2, PFK, PKM, and LDHA (G, I) in the liver of the embryos in ovo injected with 20 mg/kg 2-DG or 10 nM DAPT in the yolk sac (n = 6)