Fig. 1

The binding activity of HK010 to both 4-1BB and PD-L1. A Structure overview of HK010. B Binding of HK010 to human 4-1BB and PD-L1 at the indicated concentrations by SPR assay. C Binding of HK010 to human PD-L1-expressing CHO-K1 cells measured by flow cytometry. D Binding of HK010 to human 4-1BB-expressing CHO-K1 cells measured by flow cytometry. E HK010 bound to activated CD8+ primary T cells. F Dual-antigen capture ELISA showed that HK010 could simultaneously bind 4-1BB and PD-L1. G HK010 bridged PD-L1-expressing CHO-K1 and 4-1BB-expressing CHO-K1 cells. H Comparison of the affinity of HK010 for members of the TNF receptor superfamily and the B7 family. The results are representative of three independent experiments. ***p < 0.001