Fig. 6

HK010 increases the abundance of T cells and NK cells in tumors and antitumor efficacy. A Confocal images showing the capacity of HK010 or control antibodies to bridge activated CD8+ T cells and tumor cells. CD8+ T cells were stained red using anti-CD3 antibody, and HCC1954 cells were stained green using HK010 or control antibodies and secondary antibody. Data are representative of two repeat experiments. αPD-L1 and the combination of αPD-L1 and α4-1BB failed to induce synapse formation with T cells. B Hematoxylin and eosin (HE) staining and C multiplexed immunohistochemistry (mIHC) staining for tumor tissue treated with HK010 or control antibodies. Red and blue arrows mark tumor cell apoptosis and tumor-infiltrating lymphocytes, respectively. D The percentages of tumor-infiltrating CD3+ T, CD8+ T, CD4+ T, and NK cells in the total cells in (C) are shown. E The expression levels of Ki-67, IFNγ, CD8, CD68, and CD163 were detected in tumor tissues treated with HK010. F The percentages of tumor-infiltrating Ki-67⁺ and IFNγ⁺ cells in CD8 T and CD68⁺ and CD163⁺ cells in the total cells in (E) are shown. The values are presented as the mean ± SD from one representative of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001