Fig. 2
Verification of tsRNA sequencing data and identification of tRFtyr overexpression in LSCC samples. Hierarchical clustering (A) and volcano plots (B) of the expression profile of tsRNAs. C The top ten significant pathways identified by KEGG pathway analysis for differentially expressed tsRNA genes. D Verification of the differentially expressed transcripts by qRT–PCR in 60 paired LSCC tissues. The structure of tRFTyr (E) and tRFTyr expression in various cancers is based on MINTBase (F) and OncotRF Database (G). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, two-tailed paired t-test