Fig. 1

Design and construct of a CRISPR–dCas9–KRAB system driven by dual promoters. A Schematic diagram of the construction of CRISPR–dCas9–KRAB plasmids driven by CKM promoter and TERT promoter, respectively. This system exerts specific tumor-killing effects only in osteosarcoma cell lines. B Locations and sequences of five different sgRNAs targeting the repressed MDM2 gene