Fig. 2

The CRISPR–dCas9–KRAB system under the control of bispecific promoters acts selectively in osteosarcoma cell lines. A The relative expression of MDM2 gene in different cell lines transfected with CRISPR–dCas9–KRAB was detected by qRT–PCR. *P < 0.05, **P < 0.01. B The relative expression levels of CRISPR–dCas9 and sgRNA were detected by qRT–PCR in the normal cell line, respectively. **P < 0.01