Fig. 4

Effects of the CRISPR–dCas9–KRAB system on cell migration and invasion 24 h after transfection compared with the sgRNA control group. A wound healing assay was used to detect relative cell migration rate (%). There were statistical differences between the two groups in MG-63, Saos-2, U-2 OS, and hFOB 1.19. The P-value was less than 0.01. ANOVA was used for statistics. A Representative pictures of migration. B Statistical histograms. A transwell assay was used to detect relative cell mobility rate (%). There were statistical differences between the two groups in MG-63, Saos-2, U-2 OS, and hFOB 1.19. The P-value was less than 0.01. ANOVA was used for statistics. C Representative pictures of invasion. D Statistical histograms