Fig. 1

PI16 is downregulated in BLCA and correlates with poor patient survival. A TCGA analysis of PI16 mRNA in 19 normal and 408 BLCA tissues (P < 0.0001). B Representative images of IHC analysis of PI16 in BLCA (tumour) and adjacent normal tissue (adj.). MOD values quantified IHC. C Representative images and quantification of IHC analysis of PI16 in non-tumour bladder epithelial tissues (non-T) and stage I–IV BLCA; MOD values quantified IHC, scale bar 200 μm. D PI16 staining index (SI) distribution; SI ≥ 8, high expression. E ROC analysis of PI16 in the indicated groups. F Kaplan–Meier analysis of 68 patients with BLCA; low/high PI16 by SI/MOD cut survival info. Log-rank test calculated P value. A two-tailed t-test was used for statistical analysis. Error bars represent the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01