Fig. 2

PI16 suppresses BLCA metastasis in vivo. A Representative images of in vivo imaging system (IVIS) detection of subcutaneous tumours in nude mice (left) and their quantitation (right). B Representative images of subcutaneous tumours; the volume and weight of tumours. The indicated BLCA cells were injected subcutaneously into nude mice. C H and E staining of primary tumour borders. Scale bar 300 μm. D Representative images and quantification of microvessel density (MVD) in tumours indicated by CD31 staining. Scale bar 300 μm. E IVIS detection of lung metastases in nude mice (left) and their quantitation (right). F Bright-field images and quantification of lung metastases. G H and E of lung metastases. Scale bar 500 μm. A two-tailed t-test was used for statistical analysis. Error bars represent the mean ± SD of three independent experiments. **P < 0.01