Fig. 3

PI16 suppresses BLCA metastasis in vitro. A Representative bright-field microscopic images (left) and quantification (right) of colony formation for indicated cells. B MTS assayed PI16’s effect on indicated cell viability. ^#P < 0.01. C, D Representative images (left) and quantification (right) of migrated (C) and invaded (D) cells were analysed in a transwell assay. Scale bar 100 μm. E Representative images and quantification of tubules formed after HUVECs were cultured on matrigel-coated plates with BLCA cell conditioned medium (CM). Scale bar 100 μm. A two-tailed t-test was used for statistical analysis. Error bars represent the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01