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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO

Fig. 5

PI16 inhibits NF-κB activity by blocking ANXA1-dependent NEMO ubiquitination. A PI16-transfected T24 lysates immunoprecipitated with anti-PI16; silver stain showed PI16/ANXA1 interaction. B PI16-transfected T24 lysates immunoprecipitated with anti-PI16, anti-ANXA1 and anti-NEMO; co-IP showed PI16/ANXA1/NEMO interaction. C NF-κB luciferase reporter activity in indicated cells. D, E Western blot of K63-Ub and M1-Ub in indicated cells. F, G Anti-NEMO immunoprecipitated T24 lysates; immunoblot showed K63-Ub and M1-Ub. H Transwell migration (upper) and invasion (lower) assay images and quantitation of T24 cells. Scale bar 100 μm. I Wound healing assay images (left) and quantitation (right) of T24 cells. Scale bar 100 μm. A two-tailed t-test was used for statistical analysis. Error bars represent the mean ± SD of three independent experiments. **P < 0.01

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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