Fig. 6

PI16 inhibits NF-κB activity by blocking LUBAC expression through ANXA1 binding. A Western blot of LUBAC components in indicated cells. B Western blot of ANXA1 and LUBAC components in indicated cells. C NF-κB luciferase reporter activity in indicated cells with HOIPIN-8 (0 nM, 50 nM,100 nM, 200 nM and 400 nM, for 48 h). D, E Transwell invasion (D) and migration (E) assay images and quantitation for indicated cells with HOIPIN-8 (400 nM). Scale bar 100 μm. A two-tailed t-test was used for statistical analysis. Error bars represent the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01