Fig. 3

HOPE alleviates hepatic apoptosis in a rat model of DCD. A, B TUNEL staining and scoring of rat liver tissue (n = 6 for each group). Scale = 200 μm. C–F Western blotting and statistical analyses of BCL2, BAX, PUMA, NOXA, GADD45A, caspase-9, caspase-3, and β-actin proteins (n = 3 for each group). T-caspase indicates total caspase, C-caspase indicates cleaved caspase. G Caspase-3 activity detection (n = 6 for each group). H, I IHC staining and statistical analysis of C-caspase-3 protein in rat liver tissue (n = 6 for each group). Scale = 100 μm. ^*p < 0.05 and ^**p < 0.01