Fig. 6

JAK2/STAT3 pathway regulates the interaction between HAX1 and SERCA2b. A, B Western blotting and statistical analyses of HAX1, SERCA2b, and β-actin proteins in rat liver tissues (n = 3 for each group). C, D Western blotting and statistical analyses of HAX1, SERCA2b, and β-actin proteins after AG490 pretreatment in rat liver tissues (n = 3 for each group). E–H Western blotting and statistical analyses of JAK2, p-JAK2, STAT3, p-STAT3, HAX1, SERCA2b, and β-actin proteins after AG490 and OE-HAX1 treatment in H/R cell model of BRL-3A (n = 3 for each group). p-JAK2 indicates phospho-JAK2, p-STAT3 indicates phospho-STAT3, OE-HAX1 indicates overexpressed HAX1. I SERCA activity analysis after AG490 and OE-HAX1 treatment in H/R cell model of BRL-3A (n = 6 for each group). J CO-IP analysis of the interaction between HAX1 and SERCA2b in rat liver tissues (n = 3 for each group). K Co-staining of HAX1 (green) with SERCA2b (red) in BRL-3A cell model (n = 6 for each group). Scale = 20 μm. ^*p < 0.05 and ^**p < 0.01