Fig. 4

Genome-wide occupancy of NFATc1 at enhancers in VS. A Overall calling peaks of NFATc1 in VS of PND-25 and PND-35 with two biological repetitions. B The genomic distribution of NFATc1 peaks. C Heatmap shows the count of DEGs (left: up-regulated, right: down-regulated DEGs) within 10 kb around differentially NFATc1 binding enhancers compared between PND-25 and PND-35. D, E IGV and heatmap show the two examples of NFATc1 peak at Chr1: 168825516–168927272 (D) as well as Chr20:5798616–5800552 (E) and the followed the transcription of gene within 10 kb distance in PND-25 and PND-35. F Pearson correlation analysis shows the coefficient of correlation between differential NFATc1 binding ability at each regions and target gene transcription. Red boxes indicate the coefficient with p-value less than 0.05 while brown ones indicate p-value without statistical significance. The SG specimens from two individuals were used for ChIP sequencing